The VibroFect Device:

The Oscillatory Motion Transfection System

This device configuration enables controlled mechanical stimulation of transfection solutions, resulting in enhanced mixing compared to conventional mechanical or manual mixing methods

Key features

  • User-friendliness: plug-and-play unit requiring no setup for routine laboratory use

  • Direct operational feedback: LED status indication for operational confirmation

  • Easy maintenance: simple, accessible design with a smooth, laboratory-friendly finish for routine cleaning and maintenance

  • Minimal footprint: small benchtop size suitable for standard laboratory workspaces

  • High portability: lightweight design for easy placement and storage in the laboratory

  • Optimal stability: four rubber feet for vibration isolation and stable positioning

 

Device Components

  • Component 1: Wave Generator

Function: generates waves with controlled amplitude and frequency

Controls: adjusts frequency (100 Hz - 10 kHz) and amplitude

Output: electrical signal to power the mechanical actuator

  • Component 2: Mechanical Actuator (Wave Driver)

Function: converts input voltage into uniaxial motion along the z-axis

Design: sturdy electromechanical actuator with a drive shaft

Motion Type: controlled oscillatory movement with adjustable displacement

Connection: receives electrical input from the wave generator and provides mechanical output

  • Component 3: Test Tube Housing

Function: holds laboratory test tubes and transmits oscillatory motion to samples

Design: secure mounting system for standard laboratory tubes

Coupling: direct mechanical connection to the actuator’s drive shaft

Sample Capacity: fits tubes from 0.1 mL to 2 mL

 

Device Operation and Motion Characteristics

Direction: Uniaxial vertical oscillation (z-axis)

Frequency Range: 100 Hz to 10 kHz

Amplitude Range: 200 nm to 2 mm

Waveforms: Sine, square, or triangular wave patterns

 

Operational Sequence

Sample Loading: a test tube containing the nucleic acid and the VibroFect reagent is secured in housing (Component 3)

Signal Generation: the wave generator (Component 1) produces a controlled electrical waveform

Mechanical Conversion: the actuator (Component 2) converts an electrical signal to precise mechanical oscillation

Sample Treatment: oscillatory motion is transmitted through the housing to the sample tube

Duration Control: treatment time is 10 seconds